Article: Differential regulation of synaptic transmission by adrenergic agonists via protein kinase A and protein kinase C in layer V pyramidal neurons of rat cerebral cortex.
Table 3Effects of second messengers downstream of α1- and β-adrenoceptors
Treatment
EPSP
IPSP
Amplitude (mV)
10–90% Rise time (ms)
90–10% Decay time (ms)
Half-duration (ms)
Amplitude (mV)
Phorbol ester
Control
6.1±0.9
7.9±2.2
67.2±12.5
29.2±04.7
0.4±0.2
Phorbol ester
4.4±0.7⁎⁎
7.6±1.6
51.9±11.7
30.7±5.9
0.5±0.2
n
13
12
10
13
13
Chelerythrine
Chelerythrine
6.6±0.8
3.0±0.7
30.4±8.8
12.4±3.5
0.7±0.3
Chelerythrine+phenylephrine
6.7±1.1
2.4±0.4
15.4±6.4
5.6±1.2
1.6±0.6
n
9
7
9
9
9
db-cAMP
Control
3.8±0.6
9.4±2.1
65.5±17.5
24.0±4.2
0.7±0.4
db-cAMP
5.1±0.8⁎⁎
10.0±2.2
80.4±17.5
28.3±4.7
0.5±0.4
n
19
19
19
19
19
Ro 20–1724
Control
3.0±0.4
6.9±1.3
78.3±19.9
25.6±4.8
0.2±0.1
Ro 20–1724a
4.0±0.6⁎
7.0±1.0
76.7±11.1
22.1±2.4
0.2±0.1
Ro 20–1724a
3.4±0.4
6.7±1.2
67.6±9.0
23.0±3.3
0.2±0.1
Ro 20–1724+isoproterenol
6.3±0.8⁎⁎⁎
6.9±1.4
97.7±8.5⁎⁎⁎
29.8±2.8⁎⁎⁎
0.2±0.1
n
11
10
10
10
11
H-89
Control
5.2±0.5
6.0±0.7
85.0±21.7
21.4±2.9
0.6±0.3
H-89
5.0±0.8
6.8±1.0
76.4±26.9
21.4±3.6
0.6±0.3
H-89+isoproterenol
4.9±1.3
4.5±1.4
37.8±19.5
15.1±2.9
1.3±0.4
n
7
4
6
5
7
aBefore application of isoproterenol in combination with Ro 20–1724, intensity of electrical stimulation was reduced to avoid eliciting action potentials by isoproterenol-induced enhancement of eEPSPs.⁎P<0.05.⁎⁎P<0.01.⁎⁎⁎P<0.001 (paired t-test).